The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases

Table 1 Demographical and basic clinical features of the 5 patients

Case #	Sex	Age (y)	Sample	Underlying diseases	Laboratory test				metagenomics sequencing results and specific reads(n)
Case #	Sex	Age (y)	Sample	Underlying diseases	routine blood test	CRP (mg/L)	PCT (ng/ml)	ESR (mm/h)	metagenomics sequencing results and specific reads(n)
1	Female	81	BALF	hypertension, diabetes, coronary artery diseases	*WBC 7.7110^9, NE% 69.8, NE 5.3810^9, LN% 17.5↓, LN 1.3510^9**	53	<0.05	74	*Candida albicans* (182) Staphylococcus capitis (12) Chlamydia psittaci (6) Human betaherpesvirus 5 (2)
2	Male	45	BALF	diabetes	*WBC 8.2210^9, NE% 93.1↑, NE 7.6510^9↑, LN% 4.0↓, LN 0.3310^9↓**	226	1.712	42	Chlamydia psittaci (225) Lautropia mirabilis (28) Leuconostoc lactis (19) Rothia mucilaginosa (19) Rothia dentocariosa (12) Streptococcus parasanguinis (10) Actinomyces odontolyticus (8) *Streptococcus mitis* (4)
3	Female	85	Lung tissue	none	*WBC 6.3810^9, NE% 88.9, NE 5.6710^9, LN% 8.0↓, LN 0.5110^9**	184	1.64	64	Chlamydia psittaci (48) *Staphylococcus epidermidis* (1)
4	Female	66	Lung tissue	none	*WBC 5.4710^9, NE% 69.8, NE 3.8210^9, LN% 19.4↓, LN 1.0610^9↓**	124	0.13	17	Chlamydia psittaci (205) Corynebacterium striatum (1) *Klebsiella pneumoniae* (1)
5	Female	61	Lung tissue	none	*WBC 6.8810^9, NE% 68.4, NE 4.7010^9, LN% 22.7↓, LN 1.5610^9↓**	96.7	<0.05	37	Chlamydia psittaci (2)

Abbreviations: BALF Bronchoalveolar lavage fluid, CRP C-reactive protein (normal reference range: 0–8 mg/L), ESR Erythrocyte sedimentation rate (normal < 15 mm/h);
LN Lymphocyte, PCT Procalcitonin (normal < 0.05 ng/ml), NE Neutrophil, WBC White blood cell

ISSN: 1471-2466