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Fig. 1 | BMC Pulmonary Medicine

Fig. 1

From: Pneumocystis jirovecii colonization and its association with pulmonary diseases: a multicenter study based on a modified loop-mediated isothermal amplification assay

Fig. 1

The specificity of the real-time fluorescence LAMP assay for P. jirovecii detection. a A representative result of the LAMP assay. The green curve (labeled as 1) represents the LAMP amplification plot of one positive specimen of P. jirovecii. Other flat curves near the bottom represent amplification plots of control samples, including the negative control (with water) and other common respiratory microbes such as Candida albicans, C. tropicalis, C. krusei, C. glabrata, C. parapsilosis, Aspergillus niger, Streptococcus pneumoniae, Escherichia coli, Acinetobacter baumannii, and Methicillin resistant Staphylococcus aureus (MRSA). b A representative result of 2% agarose gel electrophoresis analysis of LAMP products. M, DNA size marker; lane 1, no template control; lane 2, P. jirovecii-positive specimen. Lanes 312 are C. albicans, C. tropicalis, C. krusei, C. glabrata, C. parapsilosis, A. nige, S. pneumoniae, E. coli, A. baumannii, and Methicillin resistant S. aureus (MRSA), respectively

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BMC Pulmonary Medicine

ISSN: 1471-2466

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