Fig. 2
Comparison of the sensitivity of real-time fluorescence LAMP assay and conventional PCR using 10-fold serial dilutions of recombinant plasmid DNA containing P. jirovecii 18S rRNA gene. a Amplification plots of the real-time fluorescence LAMP assay (from 1 × 1010 copies/μl for curve 1 to for 1 × 100 copies/μl curve 11, and no template control for curve 12). b 2% agarose gel electrophoresis of LAMP products from serial dilutions of plasmid from 1 × 1010 copies/μl for lane 1 to for 1 × 100 copies/μl lane 11. M, DNA size marker. c 2% gel electrophoresis of conversional PCR products from serial dilutions of plasmid from 1 × 1010 copies/μl for lane 1 to for 1 × 100 copies/μl lane 11. M, DNA size marker