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Fig. 3 | BMC Pulmonary Medicine

Fig. 3

From: Pneumocystis jirovecii colonization and its association with pulmonary diseases: a multicenter study based on a modified loop-mediated isothermal amplification assay

Fig. 3

Comparison of different methods for detecting LAMP products. a Amplification plots of real-time fluorescence LAMP. Curve 1, no template control; curve 2, P. jirovecii-negative patient specimen; curves 3–5, P. jirovecii-positive specimens. b Direct visual detection of end-point products as white precipitate at the bottom of reaction tubes: Tube 1, no template control (no precipitate); tube 2, P. jirovecii-negative patient specimen (no precipitate); tubes 3–5, P. jirovecii-positive specimens (precipitate at the bottom). c Visual detection of end-point products mixed with SYBR Green I fluorescent dye (Thermo Fisher Scientific). Tube 1, No template control (orange color); tube 2, P. jirovecii-negative patient specimen (orange); tubes 3–5, P. jirovecii-positive patient specimens (emerald green)

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