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Fig. 3 | BMC Pulmonary Medicine

Fig. 3

From: LncRNA MIR17HG inhibits non-small cell lung cancer by upregulating miR-142-3p to downregulate Bach-1

Fig. 3

Overexpression of MIR17HG decreased miR-142 methylation and downregulated Bach-1. The effects of overexpressing MIR17HG on miR-142 gene methylation were analyzed by MSP (a). To further analyze the relationship between MIR17HG and miR-142-3p, cells were transfected with MIR17HG vector or miR-142-3p mimic. Overexpression of MIR17HG and miR-142-3p was confirmed by qPCR at 24 h post-transfection (b). The effects of overexpressing MIR17HG on miR-142-3p was analyzed by qPCR (c). Effects of overexpressing MIR17HG on the expression of Bach-1 at both mRNA and protein levels were analyzed by qPCR and western blot, respectively (d). All experiments were repeated 3 times and mean values were presented. M, methylation; U, unmethylation; *, p < 0.05

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