Fig. 3
From: Assessment of viral RNA in idiopathic pulmonary fibrosis using RNA-seq
Detection of HERV-K transcripts in human lung tissue. a Counts for HERV mapped reads and human mapped RNA-seq reads for IPF and control RNA. b Quantification of HERV-K RNA-seq mapped reads. c Strand-specific RT-PCR was performed to detect viral env transcripts and LTR expression, and the products were resolved by electrophoresis (−RT indicates no reverse transcriptase control). d Detection of HERV-K gene expression by qualitative RT-PCR using primers designed for env and LTR. The relative transcript expression levels were calculated using the ∆∆Ct method and fold change was calculated by the ∆∆Ct of IPF/∆∆Ct of control (CNTL)