Fig. 7
From: Dynamic evolution of emphysema and airway remodeling in two mouse models of COPD
Assessment of pulmonary arteriole thickness and collagen content around arterioles by Masson’s trichrome staining, HE staining and Western blot. Six- to eight-week-old male C57BL/6 J mice were exposed to CS for 6 months or CS for 6 months and an intranasal instillation of LPS on days 1 and 14. a CS or CS + LPS exposure induced pulmonary arteriole thickening and deposition of collagen around arterioles. Masson staining of lung tissues from mice in the control group, CS group and CS + LPS group after 6 months of exposure, original magnification × 200. Bar = 100 μm. HE staining of lung tissues from mice in the control group, CS group and CS + LPS group after 6 months of exposure, original magnification × 400. Bar = 50 μm. b Quantification of collagen content around pulmonary arterioles and MT% of mice sacrificed after 6-month exposure from the control group (n = 6), CS group (n = 6) and CS + LPS group (n = 6). c Assessment of collagen I and β-actin by Western blot. The whole PVDF membrane was cropped at 70 kDa and full-length blots are presented in Additional file 1: Figure S1. d Determination of protein levels of collagen I. Data are presented as the means ± SD, N = 6; #P < 0.05; ##P < 0.01 for the comparison of the CS group with the control group; *P < 0.05; ** P < 0.01; ***P < 0.001 for the comparison of the CS + LPS group with the control group. &P < 0.05; &&P < 0.01 for the comparison of the CS + LPS group with the CS group