Fig. 5

Luciferase reporter analysis of the regulate effects of NF-κB on human NOX1 promoter constructs. a The relative luciferase activity of NOX1 promoter in TNF-α-stimulated A549 cells when NF‐κB was knocked down. Control: A549 were cultured without siRNA or TNF-α, but just transfected with Vector1.TNF-α: A549 were pre-transfected with Vector 1 and then treated with TNF-α (10 ng/ml) 24 h in the absence of NF-κB/p65 siRNA. NF-κB/p65 siRNA + TNF-α: A549 were pre-transfected with NF-κB/p65 siRNA and then Vector 1, and treated with TNF-α(10 ng/ml) for an additional 24 h. Scrambled siRNA + TNF-α: compared with the last group, A549 were pre-transfected with Scrambled siRNA instead. Each bar represents the lever of NOX1 promoter activation. The values are reported as the Mean ± S.D. (n = 6 for each group). *P < 0.05, compared with Control. #P < 0.05, compared with TNF-α and Scrambled siRNA + TNF-α. b The relative luciferase activity of different promoter-reporter plasmids in TNF-α-stimulated A549 cells. Empty vector: A549 were pre-transfected with empty vector, then treated with TNF-α(10 ng/ml) 24 h or not; Vector1: A549 were pre-transfected with Vector1, then treated with TNF-α(10 ng/ml) 24 h or not; Vector2: A549 were pre-transfected with Vector2, then treated with TNF-α(10 ng/ml) 24 h or not. The values are reported as the Mean ± S.D. (n = 6 for each group). *P < 0.05, compared with Empty vector. #P < 0.05, compared with Vector1