Table 1 The primer sequences of RT-qPCR (F, forward; R, reverse)
Primer name | Primer sequences |
|---|---|
NF-κB/p65 | F: 5’-ACAACCCCTTCCAAGTTCCT-3’ R: 5’-TGGTCCCGTGAAATACACCT-3’ |
NOX1 | F: 5’-CTGGGTGGTTAACCACTGGTTT-3’ R: 5’-ACCAATGCCGTGAATCCCTAAG-3’ |
NOX2 | F: 5’-GGAGTTTCAAGATGCGTGGAAACTA-3’ R: 5’-GCCAGACTCAGAGTTGGAGATGCT-3’ |
NOX3 | F: 5’-CCATGGGACGGGTCGGATTGT-3’ R: 5’-GGGGGCAGAGGTAAGGGTGAAGG-3’ |
NOX4 | F: 5’-AGTCAAACAGATGGGATA-3’ R: 5’-TGTCCCATATGAGTTGTT-3’ |
NOX5 | F: 5’-TATGTGACCCTCCATTTGTACCC-3’ R: 5’-CATAACTATTCAAGGATGCTGGC-3’ |
DUOX1 | F: 5’-CTCACCTCTGTTCTTCCTAT-3’ R: 5’-AGAATAAAGAAACCCTGAAG-3’ |
DUOX2 | F: 5’-CCCAAACGTCCATCAACAGA-3’ R: 5’-ATCACCCCAGAAACTCCCCT-3’ |
β-actin | F: 5’-AGGGAAATCGTGCGTGACATCAAA-3’ R: 5’-ACTCATCGTACTCCTGCTTGCTGA-3’ |