Fig. 2

POU2F2 promotes the proliferation, migration, and invasion of lung cancer cells in vitro. a Immunoblot assays showed the expression level of POU2F2 in both A549 and H1299 cells transfected with vector or plv-POU2F2 plasmids. These images were analyzed with the Image J (National Institutes of Health, USA). Original image blots are presented in Additional file 3. b CCK-8 assays were conducted to detect the effects of POU2F2 overexpression on the proliferation of A549 and H1299 cells, and the OD value at 490 nm wavelength was compared. c Colony formation assays were conducted to detect the effects of POU2F2 overexpression on the proliferation of A549 and H1299 cells, and the colony number was calculated. d Transwell migration assays were conducted to detect the effects of POU2F2 overexpression on the migration of A549 and H1299 cells, and the OD value at 490 nm wavelength was detected. e Transwell invation assays were conducted to detect the effects of POU2F2 overexpression on the invasion of both A549 and H1299 cells, and the OD value at 490 nm wavelength was measured. Results are presented as mean ± SEM, *p < 0.05, **p < 0.01