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Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: POU2F2 promotes the proliferation and motility of lung cancer cells by activating AGO1

Fig. 4

POU2F2 regulated the expression of AGO1 in lung cancer cells. a The prediction by Jasper website (http://jaspar.genereg.net) showed POU2F2 could target the sequence of AGO1 promoter regions. b Immunoblot assays showed the expression level of POU2F2 in both A549 and H1299 cells transfected with the indicated plasmids or siRNAs. These images were analyzed with the Image J (National Institutes of Health, USA). Original image blots are presented in Additional file 5. c qPCR amplification of the anti-IgG or anti-POU2F2 antibody enriched three sites of promoter fragment in A549 and H1299 cells performing CHIP assays. d, e Luciferase activity of AGO1 wild and mutant in A549 and H1299 cells co-transfected with plv-POU2F2 or plv-vector plasmids was analyzed by luciferase reporter assays. The schematic diagram was shown in d, and the results were in e

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