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Fig. 5 | BMC Pulmonary Medicine

Fig. 5

From: POU2F2 promotes the proliferation and motility of lung cancer cells by activating AGO1

Fig. 5

AGO1 knockdown suppressed the proliferation, migration, and invasion of lung cancer cells. a Immunoblot assays showed the expression level of AGO1 in both A549 and H1299 cells transfected with control or AGO1 siRNAs. These images were analyzed with the Image J (National Institutes of Health, USA). Original image blots are presented in Additional file 6. b CCK-8 assays were conducted to detect the effects of AGO1 depletion on the proliferation of A549 and H1299 cells, and the OD value at 490 nm wavelength was compared. c Colony formation assays were conducted to detect the effects of AGO1 depletion on the proliferation of A549 and H1299 cells, and the colony number was shown. d Transwell migration assays were conducted to detect the effects of AGO1 depletion on the migration of A549 and H1299 cells, and the OD value at 490 nm wavelength was detected. e Transwell invation assays were conducted to detect the effects of AGO1 knockdown on the invasion of both A549 and H1299 cells, and the OD value at 490 nm wavelength was measured. Results are presented as mean ± SEM, *p < 0.05, **p < 0.01

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