Fig.1
From: ORMDL3 regulates poly I:C induced inflammatory responses in airway epithelial cells
TLR levels in ORMDL3 knockdown and over-expressing A549 cells. A549 cells were seeded at 40,000 cell/per well in 24 well culture plate. a Western Blotting images of ORMDL3 expression in knockdown and over-expressing A549 cells. Full-length blots were presented in Additional file 1: Western Bollting Images of ORMDL3. b 25 nM siRNA control and ORMDL3 siRNA were added into the cell culture medium. The cells were cultured for 48 h. c 250 ng/ml pcDNA3.1-ORMDL3 plasmid and control plasmid were added into the cell culture medium. Transcript levels of ORMDL3, TLR3 were quantified by qPCR and with GAPDH as internal control. ΔΔCT were compared with siRNA control samples. Student’s t test was calculated. ***Indicates P < 0.001. Each group contained triplicated experiments