Fig.3From: ORMDL3 regulates poly I:C induced inflammatory responses in airway epithelial cellsTime course studies in epithelial cells stimulated with poly I:C. A549 cells and NHBE cells were used for time course studies. Cells were transfected with 25 nM ORMDL3 siRNA or 250 ng/ml pcDNA3.1-ORMDL3 for 2 days, then changed to serum free medium. Cells were stimulated with 10 μg/ml poly I:C for 6, 10, 16 and 24 h. The supernatants were collected. a The IL-6 levels in ORMDL3 knockdown A549 cell samples. b The IL-8 levels in ORMDL3 knockdown A549 cell samples. c The IL-6 levels in ORMDL3 over-expressing A549 cell samples. d The IL-8 levels in ORMDL3 over-expressing A549 cell samples. e The IL-6 levels in ORMDL3 knockdown NHBE cell samples. f The IL-8 levels in ORMDL3 knockdown NHBE cell samples. g The IL-6 levels in ORMDL3 over-expressing NHBE cell samples. h The IL-8 levels in ORMDL3 over-expressing A549 cell samples. Samples were analyzed by one way ANOVA. ***Indicates P < 0.001; **indicates P < 0.01; *indicates P < 0.05, each group contained triplicated experimentsBack to article page