ORMDL3 regulates poly I:C induced inflammatory responses in airway epithelial cells

BMC Pulmonary Medicine

Table 1 The TLR3 transcript levels in ORMDL3 knockdown A549 cells during IL1b stimulation

Time points	Group	Avr. exp	Comparison	Fold change	Q value	Comparison	Fold change	Q value
0 h	Control	7.21	–			–
2 h	Control	7.40	2 h/0 control	1.15	0.35	–
4 h	Control	7.38	4 h/0 control	1.14	0.24	–
8 h	Control	7.10	8 h/0 control	0.89	0.30	–
10 h	Control	6.98	10 h/0 control	0.87	0.22	–
0 h	siRNA	7.48	–			0 h siRNA/control	1.24	0.27
2 h	siRNA	7.15	2 h/0 siRNA	0.78	0.03	2 h siRNA/control	0.83	0.26
4 h	siRNA	7.62	4 h/0 siRNA	1.06	0.59	4 h siRNA/control	1.16	0.81
8 h	siRNA	7.19	8 h/0 siRNA	0.81	0.06	8 h siRNA/control	1.12	0.67
10 h	siRNA	7.17	10 h/0 siRNA	0.79	0.06	10 h siRNA/control	1.14	0.81

Total 25 nM ORMDL3 siRNA and control were transfected into A549 cells. RNAs were extracted from the cells after IL-1B (1 ng/ml) stimulations at 0, 2, 4, 8 and 10 h. Global gene profiling was performed by using Affymetrix Human Gene 1.1 ST Arrays. 0 h, 2 h, 4 h, 8 h,10 h indicate the sample collecting time after IL-1B stimulation
Avr. Exp average expression; h: hour

ISSN: 1471-2466