Fig. 4

TUDCA inhibits BLM-induced cell proliferation in mouse lungs. In the BLM alone and BLM + TUDCA groups, mice were intratracheally injected with a single dose of BLM (3.0 mg/kg). In the TUDCA alone and BLM + TUDCA groups, mice were intraperitoneally injected with TUDCA (250 mg/kg) once a day. Mice were sacrificed at 21 d after BLM treatment. a Pulmonary Ki67-positive cells was detected by IHC. Original magnification: 400×. b Pulmonary Ki67-positive cells were counted. c Pulmonary PCNA was detected by immunoblot. d Quantitative analysis of grayscale value. All data were expressed as median (IQR) of ten lung tissues from ten different mice