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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: Long non-coding RNA GATA6-AS1 upregulates GATA6 to regulate the biological behaviors of lung adenocarcinoma cells

Fig. 2

As the cognate sense transcript of GATA6-AS1, GATA6 was low-expressed in LUAD cells. a GEPIA database showed the expression of GATA6 in 483 LUAD tissue samples and 347 corresponding normal tissue samples. b Expression of GATA6 in 35 LUAD tissues and non-tumor tissues was detected by RT-qPCR. c The mRNA expression and protein expression of GATA6 in LUAD cells and normal lung cells were detected by RT-qPCR and western blot. d RT-qPCR analysis was performed to reveal the overexpression efficiency of GATA6 in A549 and H1975 cells. e Western blot analysis showed the expression of GATA6 at the protein level in A549 and H1975 cells after upregulation of GATA6. f LncLocator database predicted the subcellular location of GATA6-AS1. g The main distribution of GATA6-AS1 in A549 and H1975 cells was determined utilizing the subcellular fractionation assay. *p < 0.05, **p < 0.01

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