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Fig. 3 | BMC Pulmonary Medicine

Fig. 3

From: Long non-coding RNA GATA6-AS1 upregulates GATA6 to regulate the biological behaviors of lung adenocarcinoma cells

Fig. 3

MiR-4530 was the downstream molecule of GATA6-AS1. a The RIP assay verified that GATA6-AS1 existed in A549 and H1975 cells. b Suitable miRNAs were selected with the help of bioinformatic tools. c, d The expressions of miR-4530 and miR-1200 in LUAD cells and normal lung cells were detected by RT-qPCR. e RT-qPCR analysis measured the overexpression efficiency of miR-4530 in A549 and H1975 cells. f GATA6 expression in A549 and H1975 cells after the transfection of miR-4530-mimics and miR-4530-mimics + pcDNA3.1/GATA6-AS1 was investigated using RT-qPCR. g MiR-4530 expression in A549 and H1975 cells transfected with pcDNA3.1/GATA6-AS1 was detected by RT-qPCR. h GATA6 expression in A549 and H1975 cells transfected with pcDNA3.1, pcDNA3.1/GATA6-AS1, or cotransfected with pcDNA3.1/GATA6-AS1 + miR-4530 mimics was detected by RT-qPCR. i The possible binding sites of miR-4530 and GATA6-AS1 or GATA6 were predicted using bioinformatic tools. j, k The dual‑luciferase reporter assay was conducted to detect the interaction of miR-4530 and GATA6-AS1 or GATA6 in A549 and H1975 cells. l The co-existence of GATA6-AS1, miR-4530 and GATA6 in RISC was validated by the RIP assay. *p < 0.05, **p < 0.01

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