Fig. 1

The expression of SNHG16/miR-128-3p/HMGB3 in a rat model of sepsis-induced ALI. Rats were treated with CLP to establish a sepsis model. a E. coli counts in the bronchoalveolar lavage fluid (BALF) and peritoneal fluid were determined by in-vitro bacterial culture. b ELISA was conducted to determine the inflammatory cytokines including IL-1β, IL-6 and TNF-α in the lung tissue homogenate. c HE staining was used for detecting the histopathological changes of lung, kidney, liver and spleen. d IHC was used for determining Neutrophil infiltration (labeled by MPO) in the lung. N = 5. e–g qRT-PCR was employed to examine the expression of SNHG16, miR-128-3p, HMGB3 in the lung tissue of septic rats at different time points (sham, 3, 6, 12, 24, 48, 72 h). NS P > 0.05; **P < 0.01; ***P < 0.001. h–j Linear regression analysis was used to analyze the relationships among SNHG16, miR-128-3p and HMGB3 in the lung tissue of septic rats at 24 h after CLP. N = 25. All experiments were repeated for three times