Fig. 5

SNHG16 up-regulated HMGB3 through competitive inhibition of miR-128-3p. a, b The base binding relationships between SNHG16 and miR-128-3p, miR-128-3p and HMGB3 were queried through the Starbase and TargetScan database. c, d The dual-luciferase reporter assay was conducted to testify the binding relationship between SNHG16 and miR-128-3p, miR-128-3p and HMGB3, NS P > 0.05; **P < 0.01; ***P < 0.001 (vs. miR-NC group); e, f RIP assay was implemented to verify the binding relationship between SNHG16, miR-128-3p and HMGB3, NS P > 0.05; **P < 0.01; ***P < 0.001 (vs. miR-NC group); BEAS-2B cells were transfected with SNHG16 overexpression plasmids and/or miR-128-3p mimics. g–i The expression of SNHG16, miR-128-3p and HMGB3 in BEAS-2B cells was monitored by qRT-PCR; j, k WB and cellular Immunofluorescence were conducted to detect the level of HMGB3 protein in BEAS-2B cells. *P < 0.05; **P < 0.01; ***P < 0.001 (vs. NC group); ^#P < 0.05; ^##P < 0.01; ^###P < 0.001 (vs. SNHG16 group). N = 3. All experiments were repeated for three times