Fig. 4

mRNA and protein expression of CGRP, NK1R, and SP in lung tissue. Fold changes in mRNA expression of CGRP (a), NK1R (b), and SP (c) at 14 and 28 days after treatment with bleomycin were detected by RT-qPCR. Immunoreactivity for CGRP (d, e), NK1R (f-g), and SP (h, i) in lung sections was measured (original magnification × 20). Quantitative analysis was performed by the integrated optical density (IOD) values using IPP software (n = 6/group). The data are expressed as the mean ± SD. *P < 0.05