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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: Long non-coding RNA CDKN2B-AS1 enhances LPS-induced apoptotic and inflammatory damages in human lung epithelial cells via regulating the miR-140-5p/TGFBR2/Smad3 signal network

Fig. 2

Knockdown of CDKN2B-AS1 increased cell viability but inhibited apoptosis and inflammation in LPS-treated BEAS-2B cells. a CDKN2B-AS1 expression was detected using qRT-PCR after transfection of si-NC, si-CDKN2B-AS1#1, si-CDKN2B-AS1#2 or si-CDKN2B-AS1#3. GAPDH was used as an internal control, and the expression of CDKN2B-AS1 in siRNA groups was relative to si-NC group (set as 1). b CDKN2B-AS1 detection was performed by qRT-PCR in control, LPS, LPS + si-NC or LPS + si-CDKN2B-AS1#1 group. GAPDH was used as an internal control, and the expression of CDKN2B-AS1 in other three groups was relative to control group (set as 1). c Cell viability was measured using CCK-8 assay in the above groups. d, e Cell apoptosis was assessed through caspase3 activity detection (d) and flow cytometry (e). fh Inflammatory cytokines were determined via ELISA. il Western blot was conducted to analyze the protein levels of ki67, c-caspase3 and NLRP3 in four groups. The number of replicates for each experiment was 3 (n = 3). One-way analysis of variance (ANOVA) followed by Tukey’s test was used for statistical analysis. **P < 0.01, ***P < 0.001

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