Fig. 3

Downregulation of TGFBR2 inhibited the LPS-induced injury in BEAS-2B cells. a, b The qRT-PCR and western blot were used for evaluating the knockdown efficiency of si-TGFBR2#1, si-TGFBR2#2 and si-TGFBR2#3. GAPDH was used as an internal control, and TGFBR2 expression in siRNA groups was relative to si-NC group (set as 1). c, d TGFBR2 mRNA and protein levels were quantified by qRT-PCR and western blot in control, LPS, LPS + si-NC and LPS + si-TGFBR2#2 groups. GAPDH was used as an internal control, and TGFBR2 expression in other three groups was relative to control group (set as 1). e–j CCK-8 assay, caspase3 viability/flow cytometry and ELISA were applied to assess cell viability (e), cell apoptosis (f, g) and inflammatory response (h–j) in control, LPS, LPS + si-NC or LPS + si-TGFBR2#2 group. k–n The detection of ki67, c-caspase3 and NLRP3 was performed by western blot in the above groups. The number of replicates for each experiment was 3 (n = 3). One-way analysis of variance (ANOVA) followed by Tukey’s test was used for statistical analysis. **P < 0.01, ***P < 0.001