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Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: Long non-coding RNA CDKN2B-AS1 enhances LPS-induced apoptotic and inflammatory damages in human lung epithelial cells via regulating the miR-140-5p/TGFBR2/Smad3 signal network

Fig. 4

CDKN2B-AS1 regulated the LPS-induced apoptotic and inflammatory damages via the positive regulation on TGFBR2. a, b The overexpression efficiency of TGFBR2 was analyzed by qRT-PCR and western blot. GAPDH was used as an internal control, and TGFBR2 expression in TGFBR2 group was relative to pcDNA group (set as 1). c, d The qRT-PCR and western blot were performed for TGFBR2 expression detection in the following groups: LPS + si-NC, LPS + si-CDKN2B-AS1#1, LPS + si-CDKN2B-AS1#1 + pcDNA, and LPS + si-CDKN2B-AS1#1 + TGFBR2. GAPDH was used as an internal control, and TGFBR2 expression in other three groups was relative to LPS + si-NC group (set as 1). ej The measurement of cell viability (e), cell apoptosis (f, g) and inflammatory response (h–j) was carried out via CCK-8 assay, caspase3 viability assay/flow cytometry and ELISA in the above groups. kn The expression levels of ki67, c-caspase3 and NLRP3 proteins in these groups were examined by western blot. The number of replicates for each experiment was 3 (n = 3). Student’s t-test and one-way analysis of variance (ANOVA) followed by Tukey’s test were used for statistical analysis. **P < 0.01, ***P < 0.001

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