Fig. 5

CDKN2B-AS1 targeted miR-140-5p and TGFBR2 was a downstream gene of miR-140-5p. a The binding sites between CDKN2B-AS1 and miR-140-5p were predicted by lncBase. b The transfection efficiency of miR-140-5p mimic was evaluated by qRT-PCR. U6 was used as an internal control, and miR-140-5p expression in miR-140-5p group was relative to miR-NC group (set as 1). c–e The binding between CDKN2B-AS1 and miR-140-5p was affirmed using dual-luciferase reporter assay (c), RIP (d) and pull-down assay (e). f The miR-140-5p level was measured in BEAS-2B cells transfected with si-NC or si-CDKN2B-AS1#1. U6 was used as an internal control, and miR-140-5p expression in si-CDKN2B-AS1#1 group was relative to si-NC group (set as 1). g The miRWalk software was applied for the bioinformatics analysis between miR-140-5p and TGFBR2. h Dual-luciferase reporter assay was conducted to validate the interaction between miR-140-5p and TGFBR2. i The miR-140-5p level was examined via qRT-PCR after transfection of anti-NC or anti-miR-140-5p. U6 was used as an internal control, and miR-140-5p expression in anti-miR-140-5p group was relative to anti-NC group (set as 1). j–k TGFBR2 mRNA and protein levels were determined through western blot following the transfection of miR-NC, miR-140-5p, anti-NC or anti-miR-140-5p. GAPDH was used as an internal control, and TGFBR2 expression in miR-140-5p or anti-miR-140-5p group was relative to miR-NC or anti-NC group (set as 1). The number of replicates for each experiment was 3 (n = 3). Student’s t-test was used for statistical analysis. ***P < 0.001