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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: LINC01089 suppresses lung adenocarcinoma cell proliferation and migration via miR-301b-3p/STARD13 axis

Fig. 2

LINC01089 directly binds to miR-301b-3p in LUAD. a The subcellular distribution of LINC01089 in A549 and H-1975 cells was detected through subcellular fractionation plus RT-qPCR. b The binding capacity between LINC01089 and 14 miRNAs in A549 cells was analyzed through RNA pull-down assay. c The binding sites between LINC01089 and miR-301b-3p were predicted via starBase. d The expression of miR-301b-3p in LUAD cell lines and BEAS-2B cells was detected. e The overexpression efficiency of miR-301b-3p in A549 and H-1975 cells was detected. f The interaction between LINC01089 and miR-301b-3p was validated after conducting luciferase reporter assay in A549 and H-1975 cells. g The expression of miR-301b-3p was revealed by RT-qPCR after LINC01089 was overexpressed in A549 and H-1975 cells. The statistical analysis of Fig. 2e–g was determined with Student’s t test. The statistical analysis of Fig. 2b and d was evaluated with one-way ANOVA. **P < 0.01

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