Fig. 3

LINC01089 facilitated STARD13 expression via competitively binding with miR-301b-3p in LUAD. a In contrast to normal tissues, low expression of STARD13 in LUAD tissues was uncovered via GEPIA database. b A significant rise of STARD13 expression in pcDNA3.1/LINC01089-transfected cells was unveiled through RT-qPCR together with western blot analyses. c STARD13 expression was revealed through RT-qPCR and western blot analyses after A549 and H-1975 cells were transfected with miR-301b-3p inhibitor. d RT-qPCR and western blot analyses validated the expression of STARD13 in LUAD cells and BEAS-2B cell. e StarBase was applied to predict the binding sites between STARD13 and miR-301b-3p. f, g The interaction among LINC01089, miR-301b-3p and STARD13 was verified by luciferase reporter and RIP assays. The statistical analysis of Fig. 3b and c was estimated with Student’s t test. The statistical analysis of Fig. 3d, f and g was determined with one-way ANOVA. *P < 0.05, **P < 0.01