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Fig. 5 | BMC Pulmonary Medicine

Fig. 5

From: XIST promotes apoptosis and the inflammatory response in CSE-stimulated cells via the miR-200c-3p/EGR3 axis

Fig. 5

EGR3 reversed the XIST downregulation-mediated suppression of apoptosis and inflammation in CSE-stimulated cells. a RT-qPCR and western blot analysis were used to evaluate the transfection efficacy of pcDNA3.1 or pcDNA3.1/EGR3 in 16HBE cells (n = 3). b Flow cytometry analysis showed the apoptosis of CSE-treated 16HBE cells in each group (n = 3). c Western blot analysis was used to measure the protein levels of Bax, Bcl-2 and cleaved caspase-3 in CSE-treated 16HBE cells under the indicated transfection conditions (n = 3). d The concentrations of IL-6, TNF-α, IL-1β and COX2 in CSE-treated 16HBE cells under the indicated treatments were measured by ELISA (n = 3). e Western blot analysis showing the protein levels of IL-6, TNF-α, IL-1β and COX2 in CSE-treated 16HBE cells in each group (n = 3). f A schematic diagram: CSE induced XIST facilitates apoptosis and inflammatory response of 16HBE cells by binding with miR-200c-3p to upregulate EGR3. *p < 0.05, **p < 0.01, ***p < 0.001

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