Fig. 1

TLR4 was upregulated in ALI mice and LPS-stimulated cells. A H&E staining was conducted to assess the lung injury degree in ALI mice (magnification: × 100). B, C The mRNA and protein expression of TLR4 in mice’s lung tissues (n = 8 per group) was detected by RT-qPCR and western blot analyses. D, Relative TLR4 expression in lung resident macrophages and neutrophils isolated from sham or ALI mice. E, F The mRNA and protein expression of TLR4 in LPS-stimulated PBECs was detected by RT-qPCR and western blot analyses. ^#P < 0.05 compared with Sham group in B–D; ^#P < 0.05 compared with control group in E, F