Fig. 4

MiR-26a-5p overexpression alleviated cell apoptosis by modulating TLR4. A RT-qPCR was conducted to detect Bax and Bcl-2 expression in lung tissues of mice (n = 8 per group). B The activity of caspase-3 in mice’s lung tissues (n = 8 per group) was determined. C Flow cytometry assay was utilized to reveal cell apoptosis rate. D Bax and Bcl-2 expression in PBECs was calculated by RT-qPCR. E The activity of caspase-3 in PBECs was detected. ^#P < 0.05 compared with Sham + AAV-Mock + AAV-vector group, ^&P < 0.05 compared with ALI + AAV-Mock + AAV-vector group, ^@P < 0.05 compared with ALI + AAV-miR-26a-5p + AAV-vector group in A, B; ^#P < 0.05 compared with control + Mock + vector group, ^&P < 0.05 compared with LPS + Mock + vector group, ^@P < 0.05 compared with LPS + miR-26a-5p + vector group in D–F