Fig. 2

MiR-590-3p was sponged by PCGEM1. A The putative downstream miRNAs of PCGEM1 was predicted using DIANA database. B RNA pull down assay revealed the binding between the predicted 5 miRNAs and PCGEM1. C Expression of two candidate targets in NSCLC cells and BEAS-2B cells were confirmed by RT-qPCR. D RT-qPCR validated miR-590-3p expression in NSCLC and adjacent tissues. E The transfection efficiencies of miR-590-3p mimics or miR-590-3p inhibitor in NSCLC cells were tested by RT-qPCR. F Predicted binding fragment of miR-590-3p and PCGEM1 was predicted from DIANA database. G The binding between PCGEM1 and miR-590-3p in NSCLC cells was further verified though luciferase reporter assay. H Expression correlation of PCGEM1 and miR-590-3p in NSCLC tissues was determined by Spearman analysis. *p < 0.05, **p < 0.01