Fig. 4

SOX11 was downstream of miR-590-3p. A Putative targets of miR-590-3p were obtained by DIANA database. B Expression levels of seven candidate targets were testified by RT-qPCR upon miR-590-3p overexpression or downregulation. C Predicted site of miR-590-3p on SOX11 was predicted with online tool TargetScan database. D Luciferase reporter assay validated binding relationship of SOX11 and miR-590-3p in NSCLC cells. E Relative enrichment of SOX11 and miR-590-3p in the Ago2 precipitated RISCs in NSCLC cells was further confirmed via RIP assay. F Effect of PCGEM1 knockdown on SOX11 expression in NSCLC cells was validated by RT-qPCR. G RT-qPCR examined PCGEM1 expression in NSCLC cells and BEAS-2B cells. H RT-qPCR examined PCGEM1 expression in NSCLC tissues and corresponding nontumor tissues. I, J Correlation between SOX11 expression and miR-590-3p (PCGEM1) expression in NSCLC tissues was determined by Spearman’s correlation analysis. *p < 0.05, **p < 0.01.