Fig. 5

PCGEM1 promoted proliferative and migrative abilities of NSCLC cells through upregulating SOX11 expression. A RT-qPCR measured SOX11 overexpression efficacy. B CCK-8 assay detected the viability of A549 cells under indicated transfection. C Proliferation of A549 cells in indicated transfection group was testified with colony formation experiment. D Cell migration and invasion in each group were assessed via transwell experiments. E Western blot analysis evaluated protein levels of EMT markers after indicated transfection. F Images of tumors dissected from the mice in the sh-NC, sh- PCGEM1, and sh-PCGEM1 + SOX11 group. G, H Tumor growth curve and tumor weight of mice in the sh-NC, sh- PCGEM1, and sh-PCGEM1 + SOX11 group. *p < 0.05, **p < 0.01