Fig. 1

Silencing of KCNQ1OT1 facilitated cell viability and suppressed cell apoptosis and inflammation in LPS-induced WI-38 cells. A Relative expression of KCNQ1OT1 was determined by quantitative real-time polymerase chain reaction (qRT-PCR) in the serum of pneumonia patients and healthy controls. ^**P < 0.01, vs. Healthy control. B Relative expression of KCNQ1OT1 was detected by qRT-PCR in WI-38 cells. ^**P < 0.01, vs. control. C Relative expression of KCNQ1OT1 was detected by qRT-PCR in WI-38 cells transfected with small interfering (si)-negative control (NC) or si-KCNQ1OT1. ^**P < 0.01, vs. si-NC. D Cell viability was detected by 3-(4, 5-Dimethyl-2-Thiazolyl)-2, 5-Diphenyl-2-H-Tetrazolium Bromide (MTT) assay in WI-38 cells. E The apoptosis rate was detected by flow cytometry in WI-38 cells. Lower left quadrant (LL): viable cells (AnnexinV −/PI −); Upper left quadrant (UL): necrotic cells (AnnexinV −/PI +); Lower right quadrant (LR): early apoptotic cells (AnnexinV +/PI −); Upper right quadrant (UR): late apoptotic cells (AnnexinV +/PI +); The apoptotic cells (%) were calculated as cells in LR + UR. F The level of TNF-α was measured by enzyme-linked immunosorbent assay (ELISA) in WI-38 cells. G The level of IL-6 was measured by ELISA in WI-38 cells. H The level of IL-1β was measured by ELISA in WI-38 cells. (D-H) ^**P < 0.01, vs. control. ^##P < 0.01, vs. LPS + si-NC