Fig. 3

Overexpression of miR-370-3p enhanced cell viability and repressed apoptosis and inflammation in LPS-induced WI-38 cells. A Relative expression of miR-370-3p was determined by quantitative real-time polymerase chain reaction (qRT-PCR) in the serum of pneumonia patients and healthy controls. ^**P < 0.01, vs. healthy control. B Relative expression of miR-370-3p was detected by qRT-PCR in WI-38 cells. ^**P < 0.01, vs. control. C The efficiency of miR-370-3p mimics and inhibitor on the expression of miR-370-3p was detected by qRT-PCR in WI-38 cells. ^**P < 0.01, vs. mimics negative control (mimics-NC). ^##P < 0.01, vs. inhibitor NC. D Cell viability was detected by 3-(4, 5-Dimethyl-2-Thiazolyl)-2, 5-Diphenyl-2-H-Tetrazolium Bromide (MTT) assay in LPS-induced WI-38 cells. E The apoptosis rate was detected by flow cytometry in LPS-induced WI-38 cells. Lower left quadrant (LL): viable cells (AnnexinV −/PI −); Upper left quadrant (UL): necrotic cells (AnnexinV −/PI +); Lower right quadrant (LR): early apoptotic cells (AnnexinV +/PI−); Upper right quadrant (UR): late apoptotic cells (AnnexinV +/PI +); The apoptotic cells (%) were calculated as cells in LR + UR. F The level of TNF-α in LPS-induced WI-38 cells was measured by enzyme-linked immunosorbent assay (ELISA). G The level of IL-6 in LPS-induced WI-38 cells was measured by ELISA. H The level of IL-1β in LPS-induced WI-38 cells was measured by ELISA. D–H ^**P < 0.01, vs. control; ^##P < 0.01, vs. LPS + mimics-NC