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Fig. 5 | BMC Pulmonary Medicine

Fig. 5

From: Silencing of long non-coding RNA KCNQ1OT1 alleviates LPS-induced lung injury by regulating the miR-370-3p/FOXM1 axis in childhood pneumonia

Fig. 5

Knockdown of KCNQ1OT1 alleviated LPS-induced damage of WI-38 cells by regulating miR-370-3p/FOXM1 axis. A Relative expression of FOXM1 was determined by quantitative real-time polymerase chain reaction (qRT-PCR) in the serum of pneumonia patients and healthy controls. **P < 0.01, vs. Healthy control. B Relative protein level of FOXM1 was detected by western blot in WI-38 cells. **P < 0.01, vs. control. C The efficiency of pcDNA-FOXM1 on the expression of miR-370-3p was detected by qRT-PCR in WI-38 cells. **P < 0.01, vs. pcDNA-negative control (NC). D Relative protein level of FOXM1 was detected by western blot in LPS-induced WI-38 cells. E Cell viability was detected by 3-(4, 5-Dimethyl-2-Thiazolyl)-2, 5-Diphenyl-2-H-Tetrazolium Bromide (MTT) assay in LPS-induced WI-38 cells. F The apoptosis rate was detected by flow cytometry in LPS-induced WI-38 cells. Lower left quadrant (LL): viable cells (AnnexinV −/PI −); Upper left quadrant (UL): necrotic cells (AnnexinV −/PI +); Lower right quadrant (LR): early apoptotic cells (AnnexinV +/PI −); Upper right quadrant (UR): late apoptotic cells (AnnexinV +/PI +); The apoptotic cells (%) were calculated as cells in LR + UR. G The level of TNF-α in LPS-induced WI-38 cells was measured by enzyme-linked immunosorbent assay (ELISA). H The level of IL-6 in LPS-induced WI-38 cells was measured by ELISA. I The level of IL-1β in LPS-induced WI-38 cells was measured by ELISA. DI **P < 0.01, vs. small interfering (si)-NC. ##P < 0.01, vs. si-KCNQ1OT1

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