Fig. 6

Knockdown of KCNQ1OT1 mitigated LPS-caused lung injury and inflammation in mice through regulating miR-370-3p/FOXM1 axis. A The efficiency of Ad-sh-KCNQ1OT1 on the expression of KCNQ1OT1 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in lung tissues of mice. ^**P < 0.01, vs. adenovirus negative control (Ad-NC). B The efficiency of antagomiR-370-3p on the expression of miR-370-3p was detected by qRT-PCR in lung tissues of mice. ^**P < 0.01, vs. antagomiR-NC. C The efficiency of Ad-FOXM1 on the expression of FOXM1 was detected by qRT-PCR in lung tissues of mice. ^**P < 0.01, vs. Ad-NC. D Relative expression of KCNQ1OT1 was determined by qRT-PCR in LPS-induced mice. E Relative expression of miR-370-3p was determined by qRT-PCR in LPS-induced mice. F Relative expression of FOXM1 was determined by qRT-PCR in LPS-induced mice. G Hematoxylin–eosin (HE) staining of lung tissues in LPS-induced mice and lung injury score (Magnification 400 × , scale bar 50 μm). H Lung wet/dry ratio. I The level of TNF-α in bronchoalveolar lavage fluid (BALF) was measured by ELISA. J The level of IL-6 in BALF was measured by ELISA. K The level of IL-1β in BALF was measured by ELISA. D–K ^**P < 0.01, vs. control. ^##P < 0.01, vs. LPS + Ad-NC. ^&P < 0.05, ^&&P < 0.01, vs. LPS + Ad-sh-KCNQ1OT1