Fig. 5

Emodin downregulated DEGS1 expression in human PASMCs under hypoxia. a Comparison and integration analysis of differential transcriptomic and proteomic analysis. Venn diagram revealed 19 genes expressed differentially at both mRNA and protein levels. b Analysis of Pearson correlation coefficient showed that differential expression of these 19 genes was significantly correlated (R = 0.76, P < 0.001). c Differential expression of these 19 genes were used to construct PPI network to screen key genes. d Western blots and quantitative analysis of DEGS1. N = 3. Dunnett’s multiple comparisons test, *P < 0.05 and **P < 0.01 versus normoxia but 0 µM emodin group; ^#P < 0.05 and ^##P < 0.01 versus hypoxia but 0 µM emodin group