Fig. 6

DEGS1 is a direct target of miR-244-5p human PASMCs. a Bioinformatic analysis was used to predict the potential miRNAs targeting DEGS1. b Human PASMCs were treated with emodin for 24 h, and then the relative expression levels of miR-244-5p, miR-143-3p and miR-875-5p were detected using RT-qPCR. c Schematic diagram of a predicted binding site of miR-244-5p in the 3’UTR-wild type of DEGS1 mRNA (3’UTR-WT) and the 3’UTR-mutant of DEGS1 mRNA (3’UTR-MUT); the luciferase activity was determined using the dual luciferase reporter system. d The expression of miR-224-5p were determined by RT-qPCR in PASMCs transfected with miR-224-5p mimics or inhibitor. e The protein expression of DEGS1 were determined by western blot in PASMCs transfected with miR-224-5p mimics or inhibitor. N = 3. **P < 0.01 versus negative control (NC) mimic groups