Fig. 2

MiR-382-5p is the downstream of circ_NEK6 in NSCLC. A Agarose gel electrophoresis analysis was used to detect the circular feature of circ_NEK6. B RT-qPCR assay was used to assess circ_NEK6 expression in cells treated with RNase R. C The expression of linear NEK6 and circ_NEK6 under ActD treatment for indicated time was detected by RT-qPCR. D, E. FISH and subcellular separation assays were used to confirm the subcellular location of circ_NEK6. F RNA pull down assay was utilized to test the binding affinity of circ_NEK6 with 13 miRNAs. G MiR-382-5p expression in NSCLC cells and 16HBE was detected by RT-qPCR assay, and relative expression of miR-382-5p in 70 pairs of NSCLC samples was examined. H The potential binding sites between circ_NEK6 and miR-382-5p were predicted by ENCORI. I. RT-qPCR assay was used to detect the overexpression efficiency of miR-382-5p mimics. J-K. Luciferase reporter and RNA pull down assays were used to confirm the association between circ_NEK6 and miR-382-5p. All results were displayed as the mean ± SD. **P < 0.01