Fig. 1

LINC00520 presented upregulation in LUAD and promoted proliferation and migration in LUAD cells. a LINC00520 level in LUAD tissues and adjacent non-tumor ones was examined through RT-qPCR. b RT-qPCR testified LINC00520 expression in 4 LUAD cells and BEAS-2B cell line. c Kaplan Meier analysis depicted the survival curve for prognosis of patients with LUAD. d RT-qPCR detected knockdown efficacy of sh-LINC00520#1/2 in LUAD cells. e CCK-8 assay was adopted to confirm viability of LUAD cells under LINC00520 silencing. f Colony formation assay assessed LUAD cell proliferation affected by LINC00520 knockdown. g, h Apoptosis rate of LUAD cells influenced by LINC00520 downregulation was analyzed by flow cytometric analysis. i Western blotting was conducted to reveal the effects of sh-LINC00520 on Bax and Bcl-2 protein levels in A549 and H1975 cells. j Migrative ability of LUAD cells was detected by wound healing experiment after LINC00520 downregulation. k Western blot analysis examined protein expression levels of EMT markers affected by LINC00520 silencing. **p < 0.01, ***p < 0.001