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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop

Fig. 2

FOXP3 transcriptionally activated LINC00520 in LUAD cells. a TFs for LINC00520 promoter were predicted though online tool PROMO. b Putative TFs that bind to LINC00520 promoter were identified by DNA pull down assay. c RT-qPCR analysis confirmed expression of putative TFs in LUAD cells. d RT-qPCR testified FOXP3 expression levels in LUAD tissues and adjacent non-tumor tissues. e The transfection efficiency of pcDNA3.1/FOXP3 or sh-FOXP3#1/2 in LUAD cells was determined by RT-qPCR. f Effects of sh-FOXP3#1/2 and pcDNA3.1/FOXP3 on LINC00520 were confirmed by RT-qPCR. g Five FOXP3 binding sites on LINC00520 promoter were predicted through online tool JASPAR. h ChIP assay verified the binding of FOXP3 protein and LINC00520 promoter. i Luciferase reporter assay validated the effect of FOXP3 overexpression on luciferase activity of pGL3 vector containing WT/MUT LINC00520 promoter. ***p < 0.001

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BMC Pulmonary Medicine

ISSN: 1471-2466

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