Fig. 10

Levels of the endothelial nitric oxide synthase (eNOS) and phosphorylated eNOS proteins and eNOS mRNA. The left column (MCT21) represents rats in the MCT21 group.; The middle column (MCT28) represents rats in the MCT28 group.; The right column (CH) represents rats in the CH group. A: eNOS protein in the MCT21 group; B: eNOS protein in the MCT28 group; C: eNOS proein in the CH group; D: peNOS protein in the MCT21 group; E: peMOS protein in the MCT28 group; F: peNOS proein in the CH group; G: eNOS mRNA in the MCT21 group; H: eNOS mRNA in the MCT28 group; I: eNOS mRNA in the CH group. Representative Western blot images showing levels of the eNOS and peNOS proteins (A-F) are presented. Sal, injected with saline; MCT, injected with monocrotaline (MCT). Air, exposed to ambient air; CH, rats exposed to chronic hypoxia; CLZ-, fed rat chow without CLZ; CLZ +, fed rat chow with CLZ. The average intensity of the Sal/CLZ- group in the MCT21 and MCT28 models and Air/CLZ- group in the CH model was set to 100%. The intensity of each sample was calculated as a percentage of the average value (relative intensity). (n) = number of rats, means ± SE