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Fig. 6 | BMC Pulmonary Medicine

Fig. 6

From: The effects of inhaling hydrogen gas on macrophage polarization, fibrosis, and lung function in mice with bleomycin-induced lung injury

Fig. 6

TGF-β1 expression. a TGF-β1 protein in the lung as examined by western blotting. Beta-actin was evaluated as a housekeeping protein. There are two TGF-β1 bands, 44 kDa and 13 kDa. The 13 kDa band represents active form. There was no difference of 13 kDa band intensity among the 4 groups. The original, full-length blot images are shown in Supplementary Information. b Immunostaining for TGF-β1 to localize protein expression in the alveoli. Black arrows indicate cells strongly positive for TGF-β1. SA, saline administration and air inhalation; SH, saline administration and hydrogen inhalation; BA, bleomycin administration and air inhalation; BH, bleomycin administration and hydrogen inhalation. c Immunostaining with IL-6 antibody was performed on lung sections obtained from mice on day 7 after bleomycin administration. Black and white arrows indicate alveolar macrophages in BA and BH groups respectively

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