Fig. 3

Regulation of IL-8 production by HB-EGF via NF-κB–mediated signaling pathway. The effect of NF-κB inhibitor on HB-EGF–mediated IL-8 mRNA expression (a) and IL-8 release (b) from BEAS-2B and HBECs. BEAS-2B and HBECs were transfected with the NF-κB-reporter plasmid and then treated with various concentrations of rhHB-EGF for 6 h. Luciferase activity was then measured (c, d). The cell culture supernatant wasn’t changed and samples was collected at 6 h. Data are from one experiment representative of three independent experiments. Results are expressed as mean ± SEM. ^#P < 0.05 versus control of BEAS-2B cells; *P < 0.05, **P < 0.01, ***P < 0.001 versus control of HBECs