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Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration

Fig. 4

Effect of both HB-EGF-BEAS-2B-CM and HB-EGF-HBE-CM on proliferation and migration of human lung fibroblasts. CCK-8 assay was measured fibroblasts proliferation. Fibroblasts were cultured with various concentrations of HB-EGF-BEAS-2B (a) and HB-EGF-HBE-CM (b) for 24 h and 48 h. Besides, we used the transwell migration assay to assess the migration capability of fibroblasts. Lung fibroblasts were stimulated with 30% CM of BEAS-2B cells (c, d) and HBECs (e, f) for 24 h. Data are from one experiment representative of three independent experiments. Results are expressed as mean ± SEM. #P < 0.05, ##P < 0.01, ###P < 0.001 versus control medium of BEAS-2B cells; **P < 0.01, ***P < 0.001 versus control medium of HBECs

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