Fig. 2
Circ_MACF1 affects the functional behaviors and gefitinib sensitivity of A549/GR and PC9/GR cells. A549/GR and PC9/GR cells were transfected with negative control Vector plasmid or circ_MACF1 overexpression plasmid. A, B qRT-PCR analysis of circ_MACF1 and MACF1 mRNA in transfected A549/GR and PC9/GR cells. C, D MTT assay of cell viability and the IC50 values for gefitinib in transfected A549/GR and PC9/GR cells. E Representative images depicting a cell proliferation assay and cell proliferation by EdU assay. F Representative images showing a cell colony formation assay in transfected A549/GR and PC9/GR cells. G, H MTT assay for proliferation ability of transfected A549/GR and PC9/GR cells. I Representative images describing a cell apoptosis assay and flow cytometry for cell apoptosis. J, K Representative images depicting cell migration and invasion assays performed by flow cytometry. L, M Western blot showing Twist1 and E-cadherin levels in transfected A549/GR and PC9/GR cells. *P < 0.05