Fig. 3

Circ_MACF1 directly targets miR-942-5p. A Sequence of miR-942-5p, the putative complementary sequence for miR-942-5p within circ_MACF1, and the mutation in the miR-942-5p target region. B, C Dual-luciferase reporter assays in A549/GR and PC9/GR cells co-transfected with miR-942-5p mimic or miR-NC control mimic and circ_MACF1 wild-type (circ_MACF1-WT) or mutant-type (circ_MACF1-MUT) luciferase reporters. D qRT-PCR analysis showing miR-942-5p expression in A549/GR and PC9/GR cells transfected by negative control Vector plasmid or circ_MACF1 overexpression plasmid. E qRT-PCR analysis of miR-942-5p expression in A549/GR, A549, PC9/GR, and PC9 cells. *P < 0.05