Fig. 4
Circ_MACF1 overexpression affects the functional behaviors and gefitinib sensitivity of A549/GR and PC9/GR cells by down-regulating miR-942-5p. A549/GR and PC9/GR cells were transfected with negative control Vector plasmid, circ_MACF1 overexpression plasmid, circ_MACF1 overexpression plasmid + miR-NC mimic, or circ_MACF1 overexpression plasmid + miR-942-5p mimic. A Relative miR-942-5p expression by qRT-PCR analysis in transfected A549/GR and PC9/GR cells. B MTT assay for the IC50 value for gefitinib in transfected A549/GR and PC9/GR cells. C Cell proliferation by EdU assay. D Cell colony formation by colony formation assay. E, F MTT assay for cell proliferation. G Apoptosis of transfected A549/GR and PC9/GR cells by flow cytometry. H, I Transwell assay for cell migration and invasion abilities in transfected A549/GR and PC9/GR cells. J, K Western blot showing the expression levels of Twist1 and E-cadherin in transfected A549/GR and PC9/GR cells. *P < 0.05