Fig. 5

Circ_MACF1 targets miR-942-5p to regulate TGFBR2 expression. A Sequence of miR-942-5p, the putative complementary region for miR-942-5p within TGFBR2 3’UTR, and the mutation in the seed region of the miR-942-5p target sequence. B, C Dual-luciferase reporter assays in A549/GR and PC9/GR cells co-transfected with miR-942-5p mimic or miR-NC control mimic and TGFBR2 3’UTR wild-type (TGFBR2-WT) or mutant-type (TGFBR2-MUT) luciferase reporters. Relative miR-942-5p expression by qRT-PCR analysis (D), TGFBR2 mRNA level by qRT-PCR analysis (E), TGFBR2 protein level by western blot (F), in A549/GR and PC9/GR cells transfected by miR-NC mimic, miR-942-5p mimic, anti-miR-NC, or anti-miR-942-5p. TGFBR2 mRNA level by qRT-PCR analysis and TGFBR2 protein level by western blot in A549/GR, A549, PC9/GR, and PC9 cells (G, H), A549/GR and PC9/GR cells transfected by negative control Vector plasmid, circ_MACF1 overexpression plasmid, circ_MACF1 overexpression plasmid + miR-NC mimic, or circ_MACF1 overexpression plasmid + miR-942-5p mimic (I, J). *P < 0.05